The Central Scotland Green Network covers about 6,500km2. It includes a large number of derelict industrial sites that will be developed into woodland, green spaces, footpaths and cycle routes over the next 25 years.
The project is incorporated in the Scottish Government's national planning framework and will be integrated into the development plans of 19 local authorities.
A partnership board with representatives from local councils, health boards, the Forestry Commission Scotland and Scottish Natural Heritage is being established to oversee the development.
Scottish environment minister Roseanna Cunningham predicted that the network will deliver real change. "The project will improve the health of local people and give businesses a competitive edge," she said.
Forestry Commission Scotland head of policy Simon Hodge said the next stage is to identify priority sites.