The consultancy has been appointed to provide a retail study and planning services for the proposed superstore at the junction of Queensway and West Mains Road.
The scheme would create 300 jobs and bring back into use a brownfield site that has been vacant since 1998. Scottish Enterprise has sought to find an alternative industrial use for the site for more than ten years without success.
Three supermarket chains - Asda, Sainsbury's and Tesco - have all expressed interest in taking over the store but no agreement has yet been confirmed.
Knight Frank claimed that the site's location would bring in commuters as well as local residents. It lies opposite the Queensway Retail Park and the nearby Mall and Plaza shopping centres already attract consumers to the area.
Glasgow office partner David Innes said: "The two main food store operators, Asda and Tesco, do not have a store in the town. This proposal allows retail spending to be retained in East Kilbride and will also create jobs for the community."