Last week the council's cabinet adopted a motion to formally object to Transport for London's (TfL) light rail link from Shepherds Bush to Uxbridge.
The decision, expected to be ratified by the full council today, will leave Ealing as the only borough supporting the plans.
Hillingdon cabinet member for planning and transport Keith Burrows said the council's "significant concerns" have not been resolved by TfL. "A key issue for us is for the tram to run to Hayes station in the first phase, but this request has fallen on deaf ears," he said. Burrows added that the council is also concerned about rat-running and the location of the Uxbridge terminus.
Hammersmith and Fulham is opposed to the tram because of a construction compound proposed on Shepherds Bush Green (Planning, 4 November, p1).
But TfL insisted that it is committed to a scheme that "best meets the needs of all residents and businesses" in the area.