The company has objected to the London Borough of Barnet's plans for the compulsory purchase and demolition of its cash-and-carry outlet to make way for the project.
Bestway claims that there are major flaws in the council's proposals, including a letter from the North London Waste Authority stating that the site is not needed.
Last week Bestway wrote to Barnet asking for a meeting with the developers and the waste authority, but as Planning went to press it had not received a response.
"There are countless reasons why the scheme is unlawful. They are attempting to take away a very successful cash-and-carry business," said Bestway head of property Malcolm Carter.
Communities secretary Eric Pickles gave a green light to the proposals in June when he chose not to call them in (Planning, 25 June, p2). The scheme includes road improvements, schools, a train station, work to Brent Cross tube station, bridges and open spaces.
A Barnet spokesman said the council is aware of a possible legal challenge by Bestway against the waste handling facility and plant but this would not normally go ahead until it issues a relevant planning permission.
He accepted that the regeneration will affect a number of existing businesses and their needs must be fully considered in terms of relocation possibilities.